Contents

1 Example 1: Protein Data

This data set is from protein expression data captured for 39 proteins. It has two batches and two conditions corresponding to case and control.

library(BatchQC)
data(protein_data)
data(protein_sample_info)
se_object <- BatchQC::summarized_experiment(protein_data, protein_sample_info)

2 Example 2: Signature Data

This data set is from signature data captured when activating different growth pathway genes in human mammary epithelial cells (GEO accession: GSE73628). This data consists of three batches and ten different conditions corresponding to control and nine different pathways

data(signature_data)
data(batch_indicator)
se_object <- BatchQC::summarized_experiment(signature_data, batch_indicator)

3 Example 3: Bladderbatch Data

This data set is from bladder cancer data. This dataset has 57 bladder samples with 5 batches and 3 covariate levels (cancer, biopsy, control). Batch 1 contains only cancer, 2 has cancer and controls, 3 has only controls, 4 contains only biopsy, and 5 contains cancer and biopsy. This data set is from the bladderbatch package which must be installed to use this data example set (Leek JT (2023). bladderbatch: Bladder gene expression data illustrating batch effects. R package version 1.38.0).

se_object <- BatchQC::bladder_data_upload()

Session info

## R version 4.4.0 RC (2024-04-16 r86468)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 22.04.4 LTS
## 
## Matrix products: default
## BLAS:   /home/biocbuild/bbs-3.20-bioc/R/lib/libRblas.so 
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_GB              LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: America/New_York
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] BatchQC_2.1.1    BiocStyle_2.33.0
## 
## loaded via a namespace (and not attached):
##   [1] RColorBrewer_1.1-3          ggdendro_0.2.0             
##   [3] jsonlite_1.8.8              magrittr_2.0.3             
##   [5] magick_2.8.3                NCmisc_1.2.0               
##   [7] farver_2.1.1                rmarkdown_2.26             
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##  [11] memoise_2.0.1               DelayedMatrixStats_1.27.0  
##  [13] EBSeq_2.3.0                 tinytex_0.51               
##  [15] htmltools_0.5.8.1           S4Arrays_1.5.0             
##  [17] BiocNeighbors_1.23.0        SparseArray_1.5.3          
##  [19] sass_0.4.9                  KernSmooth_2.23-22         
##  [21] bslib_0.7.0                 htmlwidgets_1.6.4          
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##  [33] R6_2.5.1                    fastmap_1.1.1              
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##  [61] gplots_3.1.3.1              MASS_7.3-60.2              
##  [63] DelayedArray_0.31.1         bluster_1.15.0             
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## [103] knitr_1.46                  bookdown_0.39              
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## [117] UCSC.utils_1.1.0            lazyeval_0.2.2             
## [119] yaml_2.3.8                  evaluate_0.23              
## [121] codetools_0.2-20            RcppEigen_0.3.4.0.0        
## [123] tibble_3.2.1                BiocManager_1.30.23        
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## [127] xtable_1.8-4                munsell_0.5.1              
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## [131] GenomeInfoDb_1.41.0         tidyverse_2.0.0            
## [133] png_0.1-8                   XML_3.99-0.16.1            
## [135] parallel_4.4.0              ggplot2_3.5.1              
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